MOTS-c

A 16-residue peptide encoded within mitochondrial 12S rRNA. Studied as a regulator of metabolic homeostasis via AMPK, and as a mitochondrial-to-nuclear signalling molecule.

Preclinical evidence onlyView MOTS-c in catalog →
Total citations4
Human clinical studies1
Animal models2
In vitro studies1
Reviews / meta-analyses0

Mechanism of action

MOTS-c (MRWQEMGYIFYPRKLR) is encoded in the mitochondrial genome. Lee and colleagues showed it targets the folate–methionine cycle, causing accumulation of AICAR and consequent AMPK activation, which shifts substrate use and improves insulin sensitivity in mice.

Kim and colleagues subsequently demonstrated that under metabolic stress it translocates to the nucleus and regulates nuclear gene expression, including antioxidant response element and stress-response genes — establishing bidirectional mitochondrial-to-nuclear signalling.

Peer-reviewed literature

Study type is tagged on every entry so animal and in-vitro findings are never mistaken for human clinical evidence.

Animal model

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Lee C, et al. · Cell Metabolism · 2015

The originating paper: identified MOTS-c and showed AMPK-mediated improvement in insulin sensitivity in mice.

In vitro

The Mitochondrial-Encoded Peptide MOTS-c Translocates to the Nucleus to Regulate Nuclear Gene Expression in Response to Metabolic Stress

Kim KH, et al. · Cell Metabolism · 2018

Established nuclear translocation and regulation of stress-response gene programmes.

Animal model

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Reynolds JC, et al. · Nature Communications · 2021

Reported effects on physical capacity in mice alongside measurements in human samples.

Human clinical

Acute endurance exercise stimulates circulating levels of mitochondrial-derived peptides in humans

von Walden F, et al. · Journal of Applied Physiology · 2021

Observational human study showing endogenous MOTS-c rises acutely after endurance exercise.

Limitations & open questions

Human data is limited to observational measurement of endogenous circulating levels, for example after exercise. No human administration trials have been verified.

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